Research Assistant
Research Division
Your role is to conduct experiments assigned by the Senior Scientist. You must possess the relevant scientific knowledge required, strong communication skills to work with your team, and a pro-active mindset for continuous improvement.
Job Description:
- Tool generation – expression vector construction and molecular cloning, protein expression, stable cell lines development
- Drug screening – ELISA, FACS, cell-based functional assay, confocal microscopy, SDS-PAGE, Western Blotting, qPCR
- Cell culture – Suspension cell culture and adherent cell culture, 3D cell culture models, such as spheroid, organoid, organ slice and organ-on-a-chip
- Lab management
Requirements:
- Degree in Biomedical Science/Biotechnology/Molecular Biotechnology or a related field with at least 1-2 years of related working experience.
- Familiar with basic laboratory procedures especially mammalian cell culture.
- Experience in Molecular Biology, Protein Chemistry, 3D Cell culture, Cell engineering, Flow Cytometry, Confocal Microscopy, NGS analysis and other relevant skills will be an added advantage.
- In vivo sample handling experience is plus but not necessary.
- Programming skill ,for example Python, is also plus.
- Working experience in either industry or academia is welcome.
Job Description
- Peptide screening – ELISA, bead-based separation etc.
- DNA/RNA analysis – primer design, PCR, qPCR, purification (spin column, magnetic beads, ethanol precipitation, phenol chloroform extraction etc.), electrophoresis (Agarose gel, Urea-PAGE), NGS(Illumina) etc.
- Protein analysis – SDS-PAGE, western blotting, ELISA etc.
Requirements
The applicant is recommended to have experience in at least one of the following areas:
- Lead identification screening in drug discovery
- Antibody, protein or peptide engineering
- Biopanning, such as phage display, SELEX, in vitro compartmentalization, microfluidics
- RNA-related analytics, protein purification, NGS data analysis, Biomek operation, etc.
- Fresh graduates are welcome to apply
The applicant must have a relevant degree in Biomedical Science/Biochemistry/Chemical Biology/Biotechnology/Molecular Biotechnology.
